Enhancing Nutrients Content of Leucaena leucocephala By Solid-state Fermentation

Abstract

Leucaena leucocephala, commonly known as Petai Belalang, is a protein-dense legume with high potential for animal feed; however, its moderate crude fiber content often limits its digestibility and inclusion in livestock diets. This study utilizes solid-state fermentation (SSF) with Rhizopus oligosporus as a biotechnological strategy to achieve significant fiber reduction and protein enrichment. Key parameters investigated include fermentation duration and moisture content, which are critical for optimizing microbial growth and the secretion of cellulolytic enzymes. While moisture facilitates nutrient transport, a carefully balanced timeline is necessary to prevent protein degradation and microbial autolysis. Thisresearch examines the nutritional dynamics of Leucaena leaves over a seven-day period at varying moisture levels to establish the ideal conditions for scalable, high-quality feed production.