In-vitro Comparison of Antibacterial Activities on Stingless Bee Propolis using Selected Extraction Methods

Authors

  • OHH Al-Masoodi Faculty of Dentistry, Universiti Teknologi MARA Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia
  • HB Said Gulam Khan Faculty of Dentistry, Universiti Teknologi MARA Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia
  • IH Baharuddin Faculty of Dentistry, Universiti Teknologi MARA Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia
  • IH Ismail Faculty of Dentistry, Universiti Teknologi MARA Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia

DOI:

https://doi.org/10.24191/cos.v9i2.19230

Keywords:

Malaysian propolis, Enterococcus faecalis, Ca(OH)2, Antimicrobial activity, Heterotrigona itama

Abstract

This study aimed to compare the different selected extraction methods of propolis extracts against Enterococcus faecalis (E. faecalis). Extraction of Malaysian Propolis (MP) from Heterotrigona itama, was carried out using 70% ethanol. For the selected extraction processes, such as, centrifugation-assisted extraction (CAE), vacuum-assisted extraction (VAE), and shaking-assisted extraction (SAE) methods were used. Antimicrobial activity against E. faecalis was assessed using the antibacterial susceptibility test (AST). The results showed that SAE, followed by CAE, had better antimicrobial properties as compared to those obtained by VAE. The inhibition zones for SAE, CAE, and VAE were 5.3mm, 4.67mm, and 4.16mm, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for extracted MP by SAE were 12.5 and 25 mg/mL, respectively. Furthermore, non-setting calcium hydroxide’s [Ca(OH)2] MIC and MBC were determined to be 50 and 100 mg/mL, respectively. As a result, we believe that extraction of raw MP from Heterotrigona itama using the SAE method was more effective than Ca(OH)2 against E. faecalis. 

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Published

2022-09-01

How to Cite

Al-Masoodi, O., Said Gulam Khan, H., Baharuddin, I., & Ismail, I. (2022). In-vitro Comparison of Antibacterial Activities on Stingless Bee Propolis using Selected Extraction Methods. Compendium of Oral Science, 9(2), 23–43. https://doi.org/10.24191/cos.v9i2.19230

Issue

Vol. 9 No. 2 (2022): Compendium of Oral Science

Section

Original Article

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