In Vitro Cytotoxicity Comparison of Stingless Bee Propolis and Cisplatin against ORL-48 and HGF

Authors

  • Ikmal Hisham Ismail Faculty of Dentistry, Universiti Teknologi MARA Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia
  • Syahmirul Saharuzaman Faculty of Dentistry, Universiti Teknologi MARA Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia
  • Faatin ‘Afeefah Ahmad Naqiyuddin Faculty of Dentistry, Universiti Teknologi MARA Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia
  • Amiyah Bismelah Faculty of Dentistry, Universiti Teknologi MARA Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia
  • Khor Goot Heah Faculty of Dentistry, Universiti Teknologi MARA Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia
  • Wan Iryani Wan Ismail Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Malaysia

DOI:

https://doi.org/10.24191/cos.v12i1.5648

Keywords:

antiproliferative activity, cancer cell lines, cytotoxicity, Heterotrigona itama, propolis

Abstract

Objectives: To assess the impact of Malaysian Heterotrigona itama (HI) propolis on the proliferation of oral squamous cell carcinoma cell lines (ORL-48) and Human Gingival Fibroblasts (HGF) through comparative treatment at different concentrations and time intervals. Method: The ORL-48 and HGF cell lines were treated with varying HI propolis and cisplatin concentrations. Subsequently, the 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay evaluated the in vitro cytotoxicity of HI propolis and cisplatin against ORL-48 and HGF cell lines. Result: This study discovered that HI propolis could inhibit the proliferation of ORL-48 cells in a concentration-dependent manner. From the increased treatment concentrations, the HI propolis administration to HGF cells demonstrated a proportional rise in the cell proliferation percentage of HGF cells. Nevertheless, cisplatin treatment significantly decreased the cell viability of ORL-48 cells with a half maximal inhibitory concentration (IC50) of 0.0036 mg/ml at 72 h (p < 0.05). Meanwhile, no significant reduction in cell viability was observed in HGF cells. Conclusion: Propolis-based HI exhibited in vitro cytotoxic activity against human cancer cell lines while demonstrating lower cytotoxicity towards normal cells.

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Published

2025-03-01

How to Cite

Ismail, I. H., Saharuzaman, S., Ahmad Naqiyuddin, F. ‘Afeefah, Bismelah, A., Goot Heah, K., & Wan Ismail, W. I. (2025). In Vitro Cytotoxicity Comparison of Stingless Bee Propolis and Cisplatin against ORL-48 and HGF. Compendium of Oral Science, 12(1), 65–81. https://doi.org/10.24191/cos.v12i1.5648

Issue

Vol. 12 No. 1 (2025): Compendium of Oral Science

Section

Original Article

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