SURVIVAL STUDY AND HAEMOLYSIN ACTIVITY OF Escherichia coli IN RAW AND PASTEURIZED MILK PRODUCED IN NEGERI SEMBILAN
Keywords:
Raw milk, Survival, Haemolysin, Escherichia coli, temperature, Total Plate CountAbstract
Demand for milk has increased in Malaysia due to the increased in awareness of healthy foods consumption. Hence, research of milk is crucial to ensure that it is not contaminated with Escherichia coli. This study evaluated the survival of Escherichia coli at different temperature and haemolysin activity of Escherichia coli on blood agar. A total of 8 samples of raw fresh and pasteurized milk were collected from nearby farm and market in Negeri Sembilan, Malaysia. After an overnight exposure to four different temperatures of 00C, 280C, 350C and 450C, the bacteriological test of milk was evaluated for the presence of Escherichia
coli. Overall, all raw fresh milk sampled exceeded the acceptable limit of bacterial count of 1 x 105 CFU/ml. Raw fresh milk recorded the highest count at 35oC with 4.4 x 107 CFU/ml and the lowest at 0oC with 8.3 x 104 CFU/ml. The presence of Escherichia coli was detected in 7/20(35%) of the total raw fresh milk samples. All pasteurized milk showed no presence of Escherichia coli due to the effectiveness of heat treatment. Haemolysin test showed no haemolytic activity. Milk contaminated with Escherichia coli can cause diarrheal, gastrointestinal diseases and urinary infection. Hence, it is important to study the survival rate of Escherichia coli and its pathogenicity in milk to ensure public safety.
References
Adesiyun, A. A. & Shehu, L. M. (1990). Characteristics of Strains of Escherichia coli Isolated from Locally-Fermented Milk ("Nono") in Zaria, Nigeria. Journal of Food Protection, 53(7), 574-577
Aghemwenhio, I. S., Timilehin, A. A. & Alpheus, G. A. (2017). Susceptibility of Beta-haemolyticEscherichia coli to Commonly Used Antibiotics in Selected Hospitals in Delta State, Southern Nigeria. Archives of Clinical Microbiology., 8(2).
Akond, M., Akond, M. A., Alam, S., Hassan, S. M. R., & Shirin, M. (2009). Antibiotic Resistance ofEscherichia coli isolated From Poultry and Poultry Environment of Bangladesh. Internet Journal of Food Safety, 11, 19-23
Fig 1 Appearance of Escherichia coli on Blood Agar for Haemolysin Test (a) At 0oC, (b) At 28oC (Room Temperature), (c) At 35oC and (d) At 45oC. Appeared in grey and opaque. In white moist, glistening(c) (d) Journal of Academia Vol.8, Issue 1 (2020) 66-7774
Alhelfi, N. A., Lahmer, R. A., Jones, D. L. & Williams, A. P. (2012). Survival and Metabolic Activity of Lux-Marked Escherichia Coli O157:H7 In Different Types of Milk. Journal of Dairy Research, 79,257–261
Antony, Ally & Silvester, Reshma. (2016). Comparative Evaluation of EMB Agar and Hicrome E. coli Agar for Differentiation of Green Metallic Sheen Producing Non E. coli and Typical E. coli Colonies from Food and Environmental Samples. Journal of Pure and Applied Microbiology. 10(4), 1-8
Arias, M. L., Monge-Rojas, R., Chaves, C., & Antillón, F. (2001). Effect of Storage Temperatures On Growth And Survival Of Escherichia Coli O157: H7 Inoculated In Foods From A Neotropical Environment. Revista de biología tropical, 49(2), 517-23.
Asmahan, A. A. & Warda, S. A. (2011). Incidence of Escherichia coli in raw Cow’s Milk in Khartoum State. British Journal of Dairy Sciences, 2(1), 23-26.
Bakr, W. M. K., Hazzah, W. A., & Abaza, A. F. (2011). Detection of Salmonella and Vibrio species in some seafood in Alexandria. Journal of American Science, 7(9)
Cavalieri, S. J., Bohach, G. A. & Snyder, I. S. (1984). Escherichia Coli α -Hemolysin: Characteristics and Probable Role In Pathogenicity. Microbiological Reviews 48, 326–43.
CDC (2017). Raw Milk Questions and Answers. https://www.cdc.gov/foodsafety/rawmilk/raw-milk-
questions-and-answers.html
Disassa, N., Sibhat, B., Mengistu, S., Muktar, Y., & Belina, D. (2017). Prevalence and Antimicrobial Susceptibility Pattern of E. coli O157:H7 Isolated from Traditionally Marketed Raw Cow Milk in and around Asosa Town, Western Ethiopia. Veterinary Medicine International, 2017, 1-7.
De Silva, S. A. S. D., Kanugala, K. N. A. P. & Weerakkody, N. S. (2016). Microbiological Quality of Raw Milk and Effect On Quality By Implementing Good Management Practices. Procedia Food Science, 6(2016), 92 – 96, https://doi.org/10.1016/j.profoo.2016.02.019
Doyle, M. P. & Schoeni, J. L. (1984). Survival and Growth Characteristics of Escherichia coli Associated with Hemorrhagic Colitis. Applied and Environmental Microbiology, 855-856
Dunge, A. (2016). Microbiota Found in Raw Milk. Independent Project In Biology-Bachelor Project. Retrieved from http://stud.epsilon.slu.se
El nahas, A., Mohamed, H., El barbary, H., Mohamed H. (2015). Incidence of E. Coli In Raw Milk And Its Products. Benha Veterinary Medical Journal, 29(1), 112‐117
FAO (2011). Preventing E. coli in Foods. http://www.fao.org/fileadmin/user_upload/fcc/news/1_FAO_Preventing-E.Coli-inFood_FCC_2011.06.23.pdf
FAO/WHO. (2004). Code of Hygienic Practice for Milk and Milk Products. Joint FAO/WHO Food Standards Programme-Codex Committee on Food Hygiene, 26th Session. Journal of Academia Vol.8, Issue 1 (2020) 66-7775
FDA (2018). The Dangers of Raw Milk: Unpasteurized Milk Can Pose a Serious Health Risk https://www.fda.gov/food/buy-store-serve-safe-food/dangers-raw-milk-unpasteurized-milk-can-pose-serious-health-risk
Fernández, F., Martínez, L. M. & Perea, E. J. (1999). Relationship Between Haemolysis Production and Resistance to Fluoroquinolones Among Clinical Isolates of Escherichia Coli. Journal of Antimicrobial Chemotherapy, 43(2), 277–279
Frederick, A. (2011). Escherichia Coli, Its Prevalence and Antibiotic Resistant In Malaysia: A Mini Review. Microbiology Journal, 2011. https://doi.org/10.3923/mj.2011
Garedew, L., Berhanu, A., Mengesha, D., & Tsegay, G. (2012). Identification of Gram-Negative Bacteria From Critical Control Points Of Raw And Pasteurized Cow Milk Consumed At Gondar Town And Its Suburbs, Ethiopia. BMC Public Health, 12(950).
Grosch, W., Belitz, H-D. & Schieberie, P. (2008). Milk and Dairy Products. Food Chemistry, 498-545. doi:10.1007/978-3-540-69934-7_11
Hanson, M. L., Wendorff W. L., & Houck, K. B. (2005). Effect of Heat Treatment of Milk on Activation of Bacillus Spores. Journal of Food Protection, 68(7), 1484-6.
Hemraj, V., Diksha, S., & Avneet, G. (2013). A Review on Commonly Used Biochemical Test for Bacteria. Innovare Journal of Life Science, 1(1), 1-7.
Hossain, S., Wimalasena, S. H. M. P. & Gang, J. H. (2017). Virulence Factors and Antimicrobial Resistance Pattern of Citrobacter freundii Isolated from Healthy Pet Turtles and their Environment. Asian Journal of Animal and Veterinary Advances, 12 (1), 10-16.
King, N., Lake, K., Cressey, P. & Hudson, A. (2014). Risk Profile: Shiga Toxin-Producing Escherichia
Coli In Raw Milk. Client Report Fw13026, 8.
Kumar, P., Kumar, K., Kumar, S. & Suman, S. (2014). Cow milk and Human Health. Journal of Dairy
Science and Technology, 3(3), 1-3
Kumar, R. & Prasad, A. (2010). Detection of E. coli and Staphylococcus in Milk and Milk Products in and around Pantnagar. Veterinary World, 3(11), 495-496
Magan, N., Pavlou, A. & Chrysanthakis, I. (2001). Milk-Sense: A Volatile Sensing System Recognizes Spoilage Bacteria and Yeasts In Milk. Sensors and Actuators B, 72(2001), 28-34,
Matthew A. C., Robyn, J. L., Roland, S., Kristie, M. K., Marta, W., Brett, F. (2013). Recent Advances in Understanding Enteric Pathogenic Escherichia coli. Clinical Microbiology Reviews, 26 (4) 822- 880; doi: 10.1128/CMR.00022-13
Mourad, G., Bettache, G. & Samir, M. (2014). Composition and Nutrional value of Raw Milk. Biological Sciences and Pharmaceutical Research, 2(10), 115-122, doi: 10.15739/ibspr.005}
Nguyen, M. T. (2006). The effect of temperature on the growth of the bacteria Escherichia coli DH5α. Saint
Martin’s University Biology Journal, 1. Journal of Academia Vol.8, Issue 1 (2020) 66-7776
Noor, R., Islam, Z., Munshi, S. K. & Rahman, F. (2013). Influence of Temperature on Escherichia coli Growth in Different Culture Media. Journal of Pure And Applied Microbiology, 7(2), 899-904
Nordin, Y., Kwan, S.Y., Chang, W.S., Loo, Y.Y.,Tan, C.W.,Mohd Fadzil, S.N., Ramzi, O.S.B., Kuan, C.H., Premarathne, J.M.K.J.K., Nor-Khaizura, M.A.R., New, C.Y. & Son, R (2018). Evaluation of Bacteriological Quality of Locally Produced Raw And Pasteurised Milk In Selangor, Malaysia. Food Research, 3(3), 208 – 212
O’Connell, A., Ruegg, P. L., Jordan, K., O’Brien, B., & Gleeson, D. (2016). The Effect of Storage Temperature and Duration on The Microbial Quality of Bulk Tank Milk. Journal of Dairy Science, 99(5), 3367–3374. doi:10.3168/jds.2015-10495
O’Connor C. B. (1994). Rural Diary Technology Ilca Training Manual. Addis Ababa Ethiopia: International Lifestock Research Institute, 133
Oliveira, G. B., Favarin, L., & Luchese, R. H., & Mcintosh, D. (2015). Psychrotrophic Bacteria In Milk: How Much Do We Really Know?. Brazilian Journal of Microbiology, 46(2), 313-321. 10.1590/S1517-838246220130963.
Oliver, S. P., Boor, K. J., Murphy, S. C. & Murinda, S. E. (2009). Food Safety Hazards Associated with Consumption of Raw Milk. Foodborne Pathogens and Disease, 6(7)
Ombarak, R. A., Hinenova, A., Awasthi, S. P., Iguschi, A., Shima, A., Elbagory, A. R., Yamasaki, S. (2016). Prevalence and Pathogenic Potential of Escherichia Coli Isolates From Raw Milk And Raw Milk Cheese In Egypt. International Journal of Food Microbiology, 221, 69-76
Pal, M., Bekele, T. & Feleke, A. (2012). Public Health Significance of Pasteurized Milk. Beverage and
Food World, 39, 55-56
Pal, M., Mulu, S., Tekle, M., Pinto, S. V. (2016). Bacterial Contamination of Dairy Products. Beverage &
Food World, 43(9), 40-43,
Pepperell, C., Kus, J. V., Gardam, M. A., Humar, A., & Burrows, L. L. (2002). Low-Virulence Citrobacter
Species Encode Resistance to Multiple Antimicrobials. Antimicrobial Agents Chemotherapy, 46(11),
-3560.
Pervin, S., Akter, T., Akter, A. & Muhammad, E. U. (2016). Characterization of Pathogenic Bacteria from
Raw and Pasteurized Milk of Different location of Dhaka city in Bangladesh. Scholars Academic
Journal of Biosciences, 4(5), 377-381
Sarkar, S. (2015). Microbiological Consideration: Pasteurized Milk. International Journal of Dairy
Science, 10(5), 206-218.
Short, E. C. & Kurtz, H. J. (1971). Properties of the Hemolytic Activities of Escherichia coli. Infection And
Immunity, 3(5), 678-687
Shunda, D., Habtamu, T. & Endale, B. (2013). Assessment of Bacteriological Quality of Raw Cow Milk at
Different Critical Points in Mekelle, Ethiopia. International Journal of Livestock Research, 3(3b), 42-
Journal of Academia Vol.8, Issue 1 (2020) 66-7777
Sieuwerts, S., De Bok, F. A. M., Mols, E., Van Hylckama Vlieg, J. E. T. (2008). A Simple And Fast Method
For Determining Colony Forming Units. Letters in Applied Microbiology, 47(4), 275-278
Soomro, A. H., Arain, M. A., Khaskheli, M. & Bhutto, B. (2002). Isolation of Escherichia Coli from Raw
Milk and Milk Products in Relation to Public Health Sold under Market Conditions at Tandojam.
Pakistan Journal of Nutrition, 1(3), 151-152
Syed, Z. H., Shaker, M., Gulve, R.M., & Asef Iqbal, M. (2014). Bacterial Analysis of Raw And Packed
Milk Of Beed City. Journal of Advances in Applied Sciences and Technology, 1(1), 53-58
Than, A. A. (2011). Effect of Temperatures on the Growth of Escherichia coli from Water. Universities
Research Journal 2011,4(2), 163-171
Vacheyrou, M., Normand, A.-C., Guyot, P., Cassagne, C., Piarroux, R., Bouton, Y. (2011). Cultivable
Microbial Communities in Raw Cow Milk And Potential Transfers From Stables Of Sixteen French
Farms. International Journal of Food Microbiology, 146, 253–262.
Vithanage, N. R., Dissanayake, M., Bolge, G., Palombo, E. A., Yeager, T. R., & Datta, N.
(2017). Microbiological quality of raw milk attributable to prolonged refrigeration conditions. Journal
of Dairy Research, 84(01), 92–101. doi:10.1017/s0022029916000728
Wang, G., Zhao, T. & Doyle, M. P. (1996). Survival and Growth of Escherichia coli 0157:H7 in
Unpasteurized and Pasteurized Milk. Journal of Food Protection, 60(6), 610-613
Wardhan, R., & Nain, L. (2017). Milk Borne Pathogens: Isolation and Identification Health Risk t
Population Especially Young Ones. International Journal of Applied and Physical Sciences, 3(1), 13-
Welch, R. A. (2006). The Genus Escherichia. Prokaryotes, 6, 60-71. doi 10.1007/0-387-30746-x_3
Wiking, L., Frøst, M. B., Larsen, L. B. & Nielsen, J. H. (2002). Effects of Storage Conditions on Lipolysis,
Proteolysis And Sensory Attributes In High Quality Raw Milk. Milchwissenschaft, 57(4), 190–194
World Health Organization (2018). E.coli. Retrieved from https://www.who.int/news-room/fact-
sheets/detail/e-coli
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