PHYTOCHEMICAL EVALUATION AND ANTIMICROBIAL ACTIVITY OF SELECTED PIGMENTED PLANTS: Garcinia mangostana, Clitoria ternatea, Ardisia colorata var elliptica and Syzygium cumini
Keywords:
anthocyanin, antimicrobial activity, LC-MS Q-TOF, phytochemical, pigmented plantAbstract
In recent years, the application of plant extract as natural antimicrobial and colourants in food are
gaining interest, concurrently with the increasing reports and consumer awareness on the adverse effect
of the synthetic ones. Hence, the objectives of this study were to evaluate the phytochemicals and
antimicrobial activity of the selected pigmented plants namely, Garcinia mangostana peel, Clitoria
ternatea flower, Ardisia colorata var elliptica fruit and Syzygium cumini fruit. The phytochemicals
present in the selected pigmented plants were determined based on the phytochemical screening and
liquid chromatography mass spectrometry quadrupole time-of-flight (LC-MS Q-TOF), while
antimicrobial activity analysis was carried out using the disc diffusion technique. Phytochemical
screening revealed that Garcinia mangostana exhibited the strongest indications for the presence of
flavonoid, leucoanthocyanidin, quinone, tannin and anthocyanidin than other pigmented plants studied.
Three anthocyanin namely, delphinidin-3-O-glucoside, delphinidin-3-O-galactoside and malvidin-3-O
galactoside was first time identified in A. colorata by LC-MS Q-TOF. The antimicrobial activity
analysis showed that, Garcinia mangostana peel exhibited the strongest inhibition on fungi Aspergillus
niger and gram-positive bacteria i.e. Bacillus cereus, Bacillus subtilis and Staphylococcus aureus with
minimum inhibitory zone of 6.50, 8.50, 6.70, and 7.20 mm, respectively. Therefore, our results
suggested that the antimicrobial activity of Garcinia mangostana peel was associated with their specific
phenolic compounds. From a practical point of view, Garcinia mangostana peel may be a good
candidate for functional foods and pharmaceutical applications.
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