Application of Fourier Transform Infrared (FTIR) Spectroscopy, Gas Chromatography and Chemometric Analyses for Halal Authentication in Food Products
DOI:
https://doi.org/10.24191/joa.v13i2.6201Keywords:
Halal authentication, FTIR spectroscopy, Gas chromatography, ChemometricsAbstract
The growing global demand for halal-certified foods requires the development of robust and reliable analytical methods to ensure compliance with Islamic dietary laws. Conventional testing methods are often unable to detect non-halal ingredients in complex and highly processed food matrices. This study explores the integration of Fourier transform infrared spectroscopy (FTIR), gas chromatography (GC) and chemometric analysis as advanced tools for halal authentication in food products. FTIR spectroscopy enables rapid and non-destructive analysis of food samples, while GC provides an accurate profile of fatty acids and volatile compounds. The combination of FTIR and GC data with multivariate statistical techniques, including Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA), enables the accurate identification of non-halal substances such as lard and gelatine, even at trace levels. Studies have shown high classification accuracy in distinguishing between halal and non-halal products based on spectral and chromatographic signatures. In addition, recent advances in portable analysers and cloud-based chemometric platforms have improved the feasibility of these methods for real-time verification of halal products in both industrial and regulatory settings. This article highlights recent advances, challenges and future directions in analytical halal authentication and emphasizes the potential of integrated spectroscopic and chromatographic approaches to ensure the integrity of halal food in the global supply chain.
References
Ahda, M., & Guntarti, A. (2023). Halal food analysis using FTIR spectroscopy combined with chemometrics: A review. Current Nutrition & Food Science, 19(2), 125-135.
Aspromonte, J., Mascrez, S., Eggermont, D., & Purcaro, G. (2024). Solid-phase microextraction coupled to comprehensive multidimensional gas chromatography for food analysis. Analytical and Bioanalytical Chemistry, 416(9), 2221-2246.
Azam, M. S. E., & Abdullah, M. A. (2020). Global halal industry: realities and opportunities. IJIBE (International Journal of Islamic Business Ethics), 5(1), 47-59.
Dahimi, O., Hassan, M. S., Rahim, A. A., & Abdulkarim, S. M. (2014). Differentiation of lard from other edible fats by gas chromatography-flame ionisation detector (GC-FID) and chemometrics. Journal of Food and Pharmaceutical Sciences, 2(1), 27-31.
Durak, T., & Depciuch, J. (2020). Effect of plant sample preparation and measuring methods on ATR-FTIR spectra results. Environmental and Experimental Botany, 169, 103915.
Ellahi, R. M., Wood, L. C., Khan, M., & Bekhit, A. E. D. A. (2025). Integrity challenges in halal meat supply chain: Potential industry 4.0 technologies as catalysts for resolution. Foods, 14(7), 1135.
Fajriati, I., Rosadi, Y., Rosadi, N. N., & Khamidinal, K. (2021). Detection of animal fat mixtures in meatballs using fourier transform infrared spectroscopy (FTIR Spectroscopy). Indonesian Journal of Halal Research, 3(1), 8-12.
Hafiz, W. A. (2020). Assessment of global halal market: challenges and opportunities. African Journal of Business and Economic Research, 15(4), 5.
Hashimoto, K., Badarla, V. R., & Ideguchi, T. (2021). High‐speed Fourier‐transform infrared spectroscopy with phase‐controlled delay line. Laser & Photonics Reviews, 15(1), 2000374.
Hossain, M. M., Uddin, S. M. K., Sultana, S., Wahab, Y. A., Sagadevan, S., Johan, M. R., & Ali, M. E. (2021). Authentication of halal and kosher meat and meat products: Analytical approaches, current progresses and future prospects. Critical Reviews in Food Science and Nutrition, 62(2), 285-310.
Kalogiouri, N. P., Manousi, N., Mourtzinos, I., Rosenberg, E., & Zachariadis, G. A. (2022). A rapid GC-FID method for the determination of fatty acids in walnut oils and their use as markers in authenticity studies. Food Analytical Methods, 1-11.
Lestari, D., Rohman, A., Syofyan, S., Yuliana, N. D., Abu Bakar, N. K. B., & Hamidi, D. (2022). Analysis of beef meatballs with rat meat adulteration using Fourier Transform Infrared (FTIR) spectroscopy in combination with chemometrics. International Journal of Food Properties, 25(1), 1446-1457.
Man, Y. C., Gan, H. L., NorAini, I., Nazimah, S. A. H., & Tan, C. P. (2005). Detection of lard adulteration in RBD palm olein using an electronic nose. Food chemistry, 90(4), 829-835.
Maritha, V., Harlina, P. W., Musfiroh, I., Gazzali, A. M., & Muchtaridi, M. (2022). The application of chemometrics in metabolomic and lipidomic analysis data presentation for halal authentication of meat products. Molecules, 27(21), 7571.
Miraboutalebi, S. M., Kazemi, P., & Bahrami, P. (2016). Fatty acid methyl ester (FAME) composition used for estimation of biodiesel cetane number employing random forest and artificial neural networks: A new approach. Fuel, 166, 143-151.
Mubiru, E., Shrestha, K., Papastergiadis, A., & De Meulenaer, B. (2013). Improved gas chromatography-flame ionization detector analytical method for the analysis of epoxy fatty acids. Journal of Chromatography A, 1318, 217-225.
Mukai, Y., Arahata, M., Tashima, T., Okamoto, R., & Takeuchi, S. (2021). Quantum Fourier-transform infrared spectroscopy for complex transmittance measurements. Physical Review Applied, 15(3), 034019.
Mukai, Y., Okamoto, R., & Takeuchi, S. (2022, July). Quantum Fourier-transform infrared spectroscopy in the far-infrared region. In 2022 Conference on Lasers and Electro-Optics Pacific Rim (CLEO-PR) (pp. 1-2). IEEE.
Nan, C., Yue, W., Tao, L., & Yang, X. (2021). Fourier transform infrared nano-spectroscopy: Mechanism and applications. Applied Spectroscopy Reviews, 56(7), 531-552.
Nazri, M. Z., Rashid, S. N. A. A., Malik, S. A., Karim, H. A. A., Sani, M. S. A., & Zaidel, D. N. A. (2024). The FTIR-ATR Spectroscopy and Multivariate Data Analysis (MVDA) for Halal Authentication of Animal Fatty Acids. Malaysian Journal of Chemistry, 26(6), 184-193.
Ng, P. C., Ahmad Ruslan, N. A. S., Chin, L. X., Ahmad, M., Abu Hanifah, S., Abdullah, Z., & Khor, S. M. (2022). Recent advances in halal food authentication: Challenges and strategies. Journal of Food Science, 87(1), 8-35.
Nurani, L. H., Riswanto, F. D. O., Windarsih, A., Edityaningrum, C. A., Guntarti, A., & Rohman, A. (2022). Use of chromatographic-based techniques and chemometrics for halal authentication of food products: A review. International Journal of Food Properties, 25(1), 1399–1416.
Nurrulhidayah, A. F., Man, C. Y., Rohman, A., Amin, I., Shuhaimi, M., & Khatib, A. (2013). Authentication analysis of butter from beef fat using Fourier Transform Infrared (FTIR) spectroscopy coupled with chemometrics. International Food Research Journal, 20(3), 1383.
Pavlidis, D. E., Mallouchos, A., Ercolini, D., Panagou, E. Z., & Nychas, G. J. E. (2019). A volatilomics approach for off-line discrimination of minced beef and pork meat and their admixture using HS-SPME GC/MS in tandem with multivariate data analysis. Meat science, 151, 43-53.
Prebihalo, S. E., Berrier, K. L., Freye, C. E., Bahaghighat, H. D., Moore, N. R., Pinkerton, D. K., & Synovec, R. E. (2018). Multidimensional gas chromatography: advances in instrumentation, chemometrics, and applications. Anal. Chem, 90(1), 505-532.
Rahayu, W. S., Rohman, A., Martono, S., & Sudjadi, S. (2018). Application of FTIR spectroscopy and chemometrics for halal authentication of beef meatball adulterated with dog meat. Indonesian Journal of Chemistry, 18(2), 376-381.
Rahmania, H., & Rohman, A. (2015). The employment of FTIR spectroscopy in combination with chemometrics for analysis of rat meat in meatball formulation. Meat science, 100, 301-305.
Rohman, A. (2016). The use of infrared spectroscopy in combination with chemometrics for quality control and authentication of edible fats and oils: A review. Applied Spectroscopy Reviews, 52(7), 589–604.
Rohman, A., & Man, Y. C. (2010). Fourier transform infrared (FTIR) spectroscopy for analysis of extra virgin olive oil adulterated with palm oil. Food research international, 43(3), 886-892.
Salleh, N. A. M., Yahaya, N., Rosli, D., Azizan, M. F., Yamin, B. M., & Hassan, M. S. (2022). Characterization of lard profile from different geographical regions of husbandries and body parts of pig using FTIR technique combined with chemometrics. International Journal on Advanced Science, Engineering and Information Technology, 12(4), 1565-1573.
Suparman, S. (2015). The use of Fourier transform infrared spectroscopy (FTIR) and gas chromatography mass spectroscopy (GCMS) for halal authentication in imported chocolate with various variants. Journal of Food and Pharmaceutical Sciences, 3(1).
Usman, I., Sana, S., Afzaal, M., Imran, A., Saeed, F., Ahmed, A., Shah, Y. A., Munir, M., Ateeq, H., Afzal, A., Azam, I., Ejaz, A., Nayik, G. A., & Khan, M. R. (2024). Advances and challenges in conventional and modern techniques for halal food authentication: A review. Food Science & Nutrition, 12(3), 1430-1443.
Windarsih, A., Bakar, N. K. A., Dachriyanus, Yuliana, N. D., Riswanto, F. D. O., & Rohman, A. (2023). Analysis of pork in beef sausages using LC-orbitrap HRMS untargeted metabolomics combined with chemometrics for halal authentication study. Molecules, 28(16), 5964.
Windarsih, A., Bakar, N. K. A., Rohman, A., Yuliana, N. D., & Dachriyanus, D. (2024). Untargeted metabolomics using liquid chromatography-high resolution mass spectrometry and chemometrics for analysis of non-halal meats adulteration in beef meat. Animal Bioscience, 37(5), 918.
Zilhadia, Z., Harahap, Y., Jaswir, I., & Anwar, E. (2022). Evaluation and characterization of hard-shell capsules formulated by using goatskin gelatin. Polymers, 14(20), 4416.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Journal of Academia

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.








