Designing CRISPR/Cas9 gene knockout system for merozoite surface protein 1 in Plasmodium knowlesi.

Abstract

Malaria cases have been steadily increased over the years and merozoite surface protein 1 of Plasmodium knowlesi allows free parasite infection on human and causes malaria. There are also estimated 249 million malaria cases in 2022 . Therefore, it is important to create a design of CRISPR/ Cas9 gene knockout system to merozoite surface protein 1 of Plasmodium knowlesi. This system functions to inhibit malaria infection that occurs through the merozoite surface protein 1 of Plasmodium knowlesi to human red blood cells.