Optimization and Validation of Gas-Chromatography Mass spectroscopy (GC-MS) and Head-Space Solid Phase Microextraction (HS-SPME) for the Analysis of the Extraction of Volatile Organic Compounds (VOCs) in Nigella Sativa Seed Oils

Abstract

Nigella Sativa. L also called black seed is a type of herbs are the members of the Ranunculaceae (Buttercup family). Moreover, Nigella Sativa. has a strong aroma and slightly bitter nutty-peppery taste used as a spice and traditional medicine as it has a religious and historical background. The samples are used to study the efficiency of the solid-phase micro-extraction (SPME) fiber and the multivariate analysis using the Response Surface Method (RSM) combined with Central Composite Design (CCD) to determined and verification of the authenticity of the sold Nigella sativa seed oils in the market.