Development of Multiplex Pcr Primers for the Detection of Waterborne Parasites

Abstract

Water is a vital resource for life to sustain ecosystems and supports human, plant, and animal growth. Water is often polluted, posing health risks from microorganisms. Parasites transmitted through contaminated water, like Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum, cause diseases like amebiasis, giardiasis, and cryptosporidiosis, leading to severe health issues and millions of deaths annually. Efforts to ensure water purity are essential to prevent waterborne parasitic infections and protect global health. Detecting waterborne diseases is challenging, hence developing multiplex primers that identify the parasite's genus is crucial for specific detection in water. These primers are to be employed in the PCR process for analyzing water samples, aiding accurate identification of the three waterborne parasites.