Enhancing antibacterial property: Tetraethyl orthosilicate influence on quaternised chitosan/PVA film
DOI:
https://doi.org/10.24191/mjcet.v7i2.1203Keywords:
Quaternized chitosan, Antibacterial, Tetraethyl orthosilicate, Polymer blending, FilmAbstract
As a food packaging material, a biofilm barricades the food from contaminants. A film formulated from biopolymers with antibacterial properties is useful and holds high potential as a food packaging alternative since biopolymers are degradable, compostable, and made from renewable resources. Chitosan has been reported to demonstrate antimicrobial properties which are efficient against gram-positive and gram-negative bacteria. However, in its pure form, chitosan has poor solubility in most solvents and is limited to acidic environments only. Thus, in this study, chitosan was quaternised using hexadecyl trimethyl ammonium bromide to enhance its hydrophilicity and its antibacterial effectiveness. A film was formulated with a blend of quaternised chitosan with polyvinyl alcohol and tetraethyl orthosilicate. The FTIR results revealed the presence of peaks around 1400 cm-1 representing the asymmetric bending of the trimethylammonium group and 950 cm-1 corresponding to the quaternary ammonium group which show the success of the quaternisation process and the crosslinking with tetraethyl orthosilicate (TEOS) results in improving the hydrophilicity of the film. By using the technique of diffusion on the disc examined against E. coli, it was observed that all formulated films regardless of the presence of TEOS have antibacterial properties. The fabricated films can be used as food packaging alternative materials.
References
Akhmetova A, Myrzakhmetov B, Wang Y, Bakenov Z, & Mentbayeva A. (2022). Development of Quaternized Chitosan Integrated with Nanofibrous Polyacrylonitrile Mat as an Anion-Exchange Membrane. ACS Omega, 7(49), 45371-45380. https://doi.org/10.1021/acsomega.2c05961
Alfuraydi, R. T., Alminderej, F. M., & Mohamed, N. A. (2022). Evaluation of Antimicrobial and Anti-Biofilm Formation Activities of Novel Poly(vinyl alcohol) Hydrogels Reinforced with Crosslinked Chitosan and Silver Nano-Particles. Polymers, 14(8), 1–25. https://doi.org/10.3390/polym14081619
Ali, A., Shahid, Md. A., Hossain, Md. D., & Islam, Md. N. (2019). Antibacterial bi-layered polyvinyl alcohol (PVA)-chitosan blend nanofibrous mat loaded with Azadirachta indica (neem) extract. International Journal of Biological Macromolecules, 138, 13–20. https://doi.org/10.1016/j.ijbiomac.2019.07.015
Andreica, B.-I., Cheng, X., & Marin, L. (2020). Quaternary ammonium salts of chitosan. A critical overview on the synthesis and properties generated by quaternization. European Polymer Journal, 139, 110016. https://doi.org/10.1016/j.eurpolymj.2020.110016
Aziz, A., Nasir, M., Zamiah, N., Shaari, K., & Syazwanie, N. (2023). Evaluation of Crosslinking Degree on the Characteristics and Performance of Membranes Formulated from Quaternized Chitosan. Scientific Research Journal, 20(1), 107–123. https://doi.org/10.24191/srj.v20i1.19060
Bi, J., Tian, C., Zhang, G., Hao, H., & Hou, H. (2021). Novel procyanidins-loaded chitosan-graft-polyvinyl alcohol film with sustained antibacterial activity for food packaging. Food Chemistry, 365(1), 130534. https://doi.org/10.1016/j.foodchem.2021.130534
Caroni, J. G., de Almeida Mattos, A. V., Fernandes, K. R., Balogh, D. T., Renno, A. C. M., Okura, M. H., Malpass, A. C. G., Ferraresi, C., Garcia, L. A., Sanfelice, R. C., & Pavinatto, A. (2021). Chitosan-based glycerol-plasticized membranes: bactericidal and fibroblast cellular growth properties. Polymer Bulletin, 78(8), 4297–4312. https://doi.org/10.1007/s00289-020-03310-4
Cheah, W.Y., Show, P.-L., Ng, I.S., Lin, G.-Y., Chiu, C.-Y., Chang, Y.-K. (2019). Antibacterial activity of quaternized chitosan modified nanofiber membrane. International Journal of Biological Macromolecules, 126, 569-577. https://doi.org/10.1016/j.ijbiomac.2018.12.193
Hefni, H. H. H., Saxena, M., Mehta, R., Bhojani, G., & Bhattacharya, A. (2022). Chitosan/polyacrylonitrile composite nanofiltration membranes: towards separation of salts, riboflavin and antibacterial study. Polymer Bulletin, 79(6), 4339–4360. https://doi.org/10.1007/s00289-021-03727-5
Kalinov, K. N., Ignatova, M. G., Manolova, N. E., Markova, N. D., Karashanova, D. B., & Rashkov, I. B. (2015). Novel antibacterial electrospun materials based on polyelectrolyte complexes of a quaternized chitosan derivative. RSC Advances, 5(67), 54517–54526. https://doi.org/10.1039/C5RA08484A
Kassim Shaari, N. Z., Sulaiman, N. A., & Rahman, N. A. (2019). Thin film composite membranes: Preparation, characterization, and application towards copper ion removal. Journal of Environmental Chemical Engineering, 7(1), 102845. https://doi.org/10.1016/j.jece.2018.102845
Ke, C. L., Deng, F. S., Chuang, C. Y., & Lin, C. H. (2021). Antimicrobial actions and applications of Chitosan. Polymers, 13(6), 904. https://doi.org/10.3390/polym13060904
Khalaji, S., Golshan Ebrahimi, N., & Hosseinkhani, H. (2021). Enhancement of biocompatibility of PVA/HTCC blend polymer with collagen for skin care application. International Journal of Polymeric Materials and Polymeric Biomaterials, 70(7), 459–468. https://doi.org/10.1080/00914037.2020.1725761
Khan, M. U. A., Yaqoob, Z., Nainar, M. A. M., Razak, S. I. A., Raza, M. A., Sajjad, A., Haider, S., & Busra, F. M. (2021). Chitosan/Poly Vinyl Alcohol/Graphene Oxide Based pH-Responsive Composite Hydrogel Films: Drug Release, Anti-Microbial and Cell Viability Studies. Polymers, 13, 3124. https://doi.org/10.3390/polym13183124
Khodaman, E., Barzegar, H., Jokar, A., & Jooyandeh, H. (2022). Production and evaluation of Physicochemical, Mechanical and Antimicrobial Properties of Chia (Salvia hispanica L.) mucilage-gelatin based Edible Films Incorporated with Chitosan Nanoparticles. Journal of Food Measurement and Characterization, 16(5), 3547–3556. https://doi.org/10.1007/s11694-022-01470-7
Li, R., Wang, Y., Xu, J., Ahmed, S., & Liu, Y. (2019). Preparation and characterization of ultrasound treated polyvinyl alcohol/chitosan/dmc antimicrobial films. Coatings, 9(9), 582. https://doi.org/10.3390/coatings9090582
Liang, Y., Zhao, Y., Sun, H., Dan, J., Kang, Y., Zhang, Q., Su, Z., Ni, Y., Shi, S., Wang, J., & Zhang, W. (2023). Natural melanin nanoparticle-based photothermal film for edible antibacterial food packaging. Food Chemistry, 401, 134117. https://doi.org/10.1016/j.foodchem.2022.134117
Lou, C.-W., Lin, M.-C., Huang, C.-H., Lai, M.-F., Shiu, B.-C., & Lin, J.-H (2022). Preparation of Needleless Electrospinning Polyvinyl Alcohol/Water-Soluble Chitosan Nanofibrous Membranes: Antibacterial Property and Filter Efficiency. Polymers, 14(5), 1054. https://doi.org/10.3390/polym14051054
Luan, F., Wei, L., Zhang, J., Tan, W., Chen, Y., Dong, F., Li Q., & Guo, Z. (2018). Preparation and Characterization of Quaternized Chitosan Derivatives and Assessment of Their Antioxidant Activity. Molecules, 23(3), 516. https://doi.org/10.3390/molecules23030516
Matica M. A., Aachmann F. L., Tøndervik A., Sletta H., & Ostafe V. (2019). Chitosan as a Wound Dressing Starting Material: Antimicrobial Properties and Mode of Action. International Journal of Molecular Sciences, 20(23), 5889. https://doi.org/10.3390/ijms20235889
Nhung, L. T. T., Kim, I. Y., & Yoon, Y. S. (2020). Quaternized Chitosan-Based Anion Exchange Membrane Composited with Quaternized Poly(vinylbenzyl chloride)/Polysulfone Blend. Polymers, 12(11), 2714. https://doi.org/10.3390/polym12112714
Omer A. M., Tamer T. M., Khalifa R. E., Eltaweil A. S., Agwa M. M., Sabra S., Abd-Elmonem M. S., Mohy-Eldin M. S., & Ziora Z. M. (2021) Formulation and Antibacterial Activity Evaluation of Quaternized Aminochitosan Membrane for Wound Dressing Applications. Polymers, 13(15), 2428. https://doi.org/10.3390/polym13152428
Sagar, S., Alturki, A., Farhan, M., Bahadar, A., & Hossain, N. (2022). Synergistic influence of tetraethyl orthosilicate crosslinker on mixed matrix hydrogels. Applied Nanoscience 12, 2923–2932. https://doi.org/10.1007/s13204-022-02606-3
Wang, Z., Ke, M., He, L., Dong, Q., Liang, X., Rao, J., Ai, J., Tian, C., Han, X., & Zhao, Y. (2021). Biocompatible and antibacterial soy protein isolate/quaternized chitosan composite sponges for acute upper gastrointestinal hemostasis. Regenerative Biomaterials, 8(4), 1-12. https://doi.org/10.1093/rb/rbab034
Wardhono, E. Y., Pinem, M. P., Susilo, S., Siom, B. J., Sudrajad, A., Pramono, A., Meliana, Y., & Guénin, E. (2022). Modification of Physio-Mechanical Properties of Chitosan-Based Films via Physical Treatment Approach. Polymers, 14(23), 5216. https://doi.org/10.3390/polym14235216
Wu, J.-Y., Wang, C.-Y., Chen, K.-H., Lai, Y.-R., Chiu, C.-Y., Lee, H.-C., & Chang, Y.-K. (2022). Electrospinning of Quaternized Chitosan-Poly(vinyl alcohol) Composite Nanofiber Membrane: Processing Optimization and Antibacterial Efficacy. Membranes, 12(3), 332. https://doi.org/10.3390/membranes12030332
Jiang, X., Cai, K., Zhang, J., Shen, Y., Wang, S., & Tian, X. (2011). Synthesis of a novel water-soluble chitosan derivative for flocculated decolorization. Journal of hazardous materials, 185(2-3), 1482–1488. https://doi.org/10.1016/j.jhazmat.2010.10.072
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