Qualitative study of Curcuma xanthorrhiza using RP-HPLC-DAD
DOI:
https://doi.org/10.24191/mjcet.v5i2.19495Keywords:
Chromatography, Curcuma, Herb, Monograph, TemulawakAbstract
The chemical analysis of Curcuma xanthorrhiza was performed via Reversed Phased High Performance Liquid Chromatography-Diode Array Detection (RP-HPLC-DAD). Prior to the investigations, the extract was obtained via maceration. The chosen HPLC mobile phase was not similar with the one published in the national herbal monograph. Instead of utilising 0.1% formic acid in water and methanol, this work involved a higher acidity of formic acid [0.3%] and acetonitrile. The chromatograms were studied, and two major peaks were observed in the stepwise gradient, with the retention times recorded at tR = 14.868 and 29.777 minutes. These signals represent two individuals, yet, unidentified constituents, since the chromatographic patterns were not satisfactory and non-comparable with the peaks in the fast, non-isocratic mode. In summary, more experiments should be conducted to understand the elution of the Curcuma’s targeted compounds, particularly xanthorrhizol and curcuminoids.
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