POTENTIAL OF AQUEOUS EXTRACTS OF Allium sativum, Mentha spicata, Myrtus communis AND Thymus vulgaris AS ANTIMICROBIALS AND CURING OF ANTIBIOTIC RESISTANT GENES IN Klebsiella pneumoniae.
Keywords:
Klebsiella pneumoniae, Allium sativum, Mentha spicata, Myrtus communis, Thymus vulgarisAbstract
Eighty six clinical isolates of Klebsiella pneumoniae were isolated from various pathogenic
cases from Erbil hospitals. The isolates were varied in their resistance to antibiotics, and
ranged between 13-18 antibiotics out of 22. Genetic transformation between purified plasmid
from K. pneumoniae and E. coli JM83 demonstrated that all tested antibiotic resistant genes
were plasmid DNA born except Doxycilin resistance gene was chromosomal born. The watery
extracts of Allium sativum, Mentha spicata Myrtus communis and Thymus vulgaris were
proved to be most powerful against K. pneumoniae K11 and K32 isolates. A comparative
evaluation of plasmids elimination from K. pneumoniae isolates by sub-MIC of plant extracts
showed that these extracts could cure plasmids effectively at their respective sub-MIC
concentration. Plasmid cured was observed by sub-MIC of Allium sativum and Thymus
vulgaris at 350 µg ml-1 cured 2, 4 and 3 plasmids, Mentha spicata and Myrtus communis
extract at 250 µg ml-1
cured 2, 3 and 2, 4 plasmids from K. pneumoniae K11 and K32 isolates.
Curing of plasmid DNA from K. pneumoniae isolates confirmed by determining the loss of
resistance markers in cured cultures.
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