ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF MIXED METHANOL EXTRACT OF ALOE VERA, HIBISCUS FLOWER AND ATI-ATI LEAVES

Authors

  • Nur Amira Mohd Amin School of Biological Sciences, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000 Kuala Pilah, Negeri Sembilan, Malaysia
  • Ida Muryany Md Yasin School of Biological Sciences, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Cawangan Negeri Sembilan, Kampus Kuala Pilah, 72000 Kuala Pilah, Negeri Sembilan, Malaysia

Keywords:

Antimicrobial, antioxidant activity, aloe vera, hibiscus flowers, ati-ati leaves

Abstract

Nowadays, modern medication always used synthetic drugs which will cause many bad effects and ignoring the safe medication such as herb plants that possess antimicrobial and antioxidant properties. The aim of the study is to evaluate the antimicrobial activity and to determine antioxidant assay of mixed methanol extract of Aloe Vera, Hibiscus flowers and Ati-ati leaves. The antimicrobial activity involved disk diffusion assay and the antioxidant activity is using DPPH radical scavenging assay. The concentration of mixed extract used were 500 mg/ml, 250 mg/ml, 100 mg/ml and 50 mg/ml. From the result of antibacterial activity, Klebsiella pneumoniae and Escherichia coli have higher inhibition zones than Bacillus cereus and Bacillus subtilis which are 22.33 mm and 16 mm respectively at higher concentration of extract, 500 mg/ml. For the antifungal activity, Candida albicans showed the highest inhibition zones than Rhizopus oligosporus and Rhizopus stolonifer which 13.66 mm at the highest concentration of the mixed extract. For the antioxidant activity, the highest concentration which 500 mg/ml have highest radical scavenging that is 93.31% while for the other concentration 250, 100 and 50 mg/ml contain 73.00%, 36.67% and 7.82% respectively of radical scavenging activity. As conclusion, mixed methanol extract of Aloe Vera, Hibiscus flowers and Ati-ati leaves have ability to conduct antimicrobial and antioxidant activity against pathogenic microbe tested.

References

References

Afiune, L. A., Leal-Silva, T., Sinzato, Y. K., Moraes-Souza, R. Q., Soares, T. S., Campos, K. E., & Volpato, G. T. (2017). Beneficial effects of Hibiscus rosa-sinensis L. Flower aqueous extract in pregnant rats with diabetes. PLOS ONE, 12(6), e0179785 https://doi.org/10.1371/journal.pone.0179785

Alzohairy, M. A. (2016). Therapeutics role of Azadirachta indica (Neem) and their active constituents in disease prevention and treatment. Evidence-Based Complementary and Alternative Medicine, 2016, 1-11. https://doi.org/10.1155/2016/7382506

Athiban, P. P., Borthakur, B. J., Ganesan, S., & Swathika, B. (2012). Evaluation of antimicrobial efficiacy of Aloe vera and its effectiveness in decontaminating gutta-percha cones. Journal of Conservative Dentistry, 15(3), 246 - 248. https://doi.org/10.4103/0972-0707.97949

Bismellah, N., Ahmad, R., Kassim, Zethy & Ismail, N. (2019). Coleus blumei extract as a potential antibacterial oral rinse. IOP Conference Series: Earth and Environmental Science, 269(1), 012-015. https://doi.org/10.1088/1755-1315/269/1/012015

Boldt, J. K., & Barrett, J. E. (2006). Response of Coleus (Solenostemon scutellarioide) to plant growth regulators applied during propagation. HortScience, 41(4), 983E - 984. https://doi.org/10.21273/hortsci.41.4.983e

Chakraborty, P. (2018). Herbal genomics as tools for dissecting new metabolic pathways of unexplored medicinal plants and drug discovery. Biochimie Open, 6, 9-16. https://doi.org/10.1016/j.biopen.2017.12.003

Dieu-Hien T., Dinh Hieu N., Nhat T. A.T., Anh V. B., Tuong H. D., & Hoang C. N., (2019). Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia. Journal of Food Quality, 2019, 1- 9. https://doi.org/10.1155/2019/8178294

Federer, C., Yoo, M., & Tan, A. C. (2016). Big Data Mining and adverse event pattern analysis in Clinical Drug Trials. ASSAY and Drug Development Technologies, 14(10), 557–566. https://doi.org/10.1089/adt.2016.742

Goldberg, K. H., Yin, A. C., Mupparapu, A., Retzbach, E. P., Goldberg, G. S., & Yang, C. F. (2017). Components in aqueous Hibiscus rosa-sinensis flower extract inhibit in vitro melanoma cell growth. Journal of Traditional and Complementary Medicine, 7(1), 45-49. https://doi.org/10.1016/j.jtcme.2016.01.005

Gourdazi, M., Fazeli, M., Azad, M., Seyedjavadi, S. S., & Mousavi, R. (2015). Aloe vera Gel: Effective therapeutic agent against multidrug-resistant pseudomonas aeruginosa isolates recovered from burn wound infections. Chemotherapy Research and Practice, 2015, 1-5. https://doi.org/10.1155/2015/639806

Jerez-Puebla, L. E. (2012). Fungal infections in immunosuppressed patient. Immunodeficiency, 149-176 https://doi.org/10.5772/51512

Kasote, D. M., Katyare, S. S., Hedge, M. V., & Bae, H. (2015). Significance of antioxidant potential of plants and its relevance to therapeutic applications. International Journal of Biological Sciences, 11(8), 982-981. https://doi.org/10.7150/ijbs.12096

Khristi, V., & Patel, V. H. (2017). Therapeutic potential of hibiscus Rosa Sinensis. International Journal of Nutrition and Dietetics, 4(2), 105-123. https://doi.org/10.17654/nd004020105

Loganayaki, N., Siddhuraju, P., & Manian, S. (2011). Antioxidant activity and free radical scavenging capacity of phenolic extracts from Helicteres isora L. and Ceiba pentandra L. Journal of Food Science and Technology, 50(4), 687-695. https://doi.org/10.1007/s13197-011-0389-x

Mai-Prochnow, A., Clauson, M., Hong, J., & Murphy, A. B. (2016). Gram positive and Gram negative bacteria differ in their sensitivity to cold plasma. Scientific Reports, 6(1), 1-11. https://doi.org/10.1038/srep38610

Mandal, M. D., & Mandal, S. (2011). Honey: Its medicinal property and antibacterial activity. Asian Pacific Journal of Tropical Biomedicine, 1(2), 154-160. https://doi.org/10.1016/s2221-1691(11)60016-6

Nair,G. R., Naidu,G. S., Jain,S., Nagi,R., Makkad,R. S., Jha,A. (2016).Clinical effectiveness of aloe vera in the management of oral mucosal diseases - A Systematic Review. Journal Clinical of Diagnostic Research. 10(8), ZE01-ZE07. https://www.doi.org/10.7860/JCDR/2016/18142/8222

Perea, S., & Patterson, T. F. (2002). Antifungal resistance in pathogenic fungi. Clinical Infection Disease, 35(9), 1073-1080. https://doi.org/10.1086/344058

Prabhakar, K., Kumar, L. S., Rajendran, S., Chandrasekaran, M., Bhaskar, K., & Sajit Khan, A. K. (2008). Antifungal activity of plant extracts against Candida species from oral lesions. Indian Journal of Pharmaceutical Sciences, 70(6), 801–803. https://doi.org/10.4103/0250-474x.49128

Rahman, M. M., Islam, M. B., Biswas, M., & Kurshid Alam, A. H. (2015). In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Research Notes, 8(1), 621-630. https://doi.org/10.1186/s13104-015-1618-6

Rahman, S., Carter, P., & Bhattarai, N. (2017). Aloe Vera for tissue engineering applications. Journal of Functional Biomaterials, 8(1), 6-23. https://doi.org/10.3390/jfb8010006

Rajeswari, D. (2016). Antioxidant and free radical scavenging capacity of extensively used medicinal plant of Punica granatum. Asian Journal of Pharmaceutical and Clinical Research, 9(6), 140-146. https://doi.org/10.22159/ajpcr.2016.v9i6.13941

Rajurkar, N. S., & Hande, S. M. (2011). Estimation of phytochemical content and antioxidant activity of some selected traditional Indian medicinal plants. Indian Journal of Pharmaceutical Sciences, 73(2), 146-151. https://doi.org/10.4103/0250-474x.91574

Siddiqui, A. A., Wani, S. M., Rajesh, R., & Alagarsamy, V. (2006). Phytochemical and pharmacological investigation of flowers of hibiscus rosasinensis Linn. Indian Journal of Pharmaceutical Sciences, 68(1), 127-130. https://doi.org/10.4103/0250-474x.22986

Subhaswaraj, P., Sowmya, M., Bhavana, V., Dyavaiah, M., & Siddhardha, B., (2017). Determination of antioxidant activity of Hibiscus sabdariffa and Croton caudatus in Saccharomyces cerevisiae model system. Journal of Food Science, and Technology, 54(9), 2728-2736. https://doi.org/10.1007/s13197-017-2709-2

Surapuram, V., William, N., Setzer, Robert, L., McFeeters and Hana McFeeters (2014). Antifungal activity of plant extracts against Aspergillus niger and Rhizopus stolonifer. Natural product Communication, 19(11), 1603-1605. https://doi.org/10.1177/1934578x1400901118

Stratton, M., Capitena, C., Christensen, L., & Paciuc-Beja, M. (2015). Escherichia coli eyelid abscess in a patient with alcoholic cirrhosis. Case Reports in Ophthlmological Medicine, 2015, 1-2. https://doi.org/10.1155/2015/827609

Zhang, L., Lin, H., Liu, W., Dai, B., Yan, L., Cao, Y. B., & Jiang, Y.-Y. (2017). Antifungal activity of the ethanol extract from flos rosae chinensis with activity against fluconazole-resistant clinical candida. Evidence-Based Complementary and Alternative Medicine, 2017, 1–10. https://doi.org/10.1155/2017/4780746

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2023-04-30

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