PHYTOCHEMICAL ANALYSIS AND BIOACTIVITY STUDIES OF ZIZIPHUS MAURITIANA (TWIGS AND LEAVES)
Keywords:
Ziziphus mauritiana, Phytochemical analysis, Thin layer chromatography, Antibacterial, AntioxidantAbstract
Malaysia lack and inadequate research about Ziziphus mauritiana. Two part of Z. mauritiana
plant obtained which are leaves and twigs for this study. Z. mauritiana extraction processes
conducted using three difference polarity of solvents which are hexane, chloroform and
methanol by cool extraction method. The highest yield percentage is chloroform leaves extract
which is 3.71%. The phytochemical analysis study revealed that many secondary metabolites
presence inside Z. mauritiana such as alkaloid, flavonoid, glycoside, phenol, saponin, steroid,
sterol, tannin and terpenoid but for leave part only absence of saponin detected. In thin layer
chromatography (TLC) method, the mixture of solvent system used are hexane and chloroform
with ratio 3:7 or 1:9 to obtain good separation of compound under short (254 nm) and long
(366 nm) wavelength UV lamp. Antibacterial activity examined using disc diffusion method
toward Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Salmonella sp. conducted.
The highest maximum zone inhibition diameter recorded is hexane leaves crude extract on S.
aureus bacteria which is 15 mm. In addition, antioxidant study discovered the highest
percentage found on crude extract of methanol.
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