EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF HALIA MERAH (Zingiber officinale var. rubrum Theilade) ETHANOLIC EXTRACT OBTAINED FROM DESA WISATA TUO PARIANGAN, WEST SUMATRA

Abstract

Halia Merah (Zingiber officinale var. rubrum Theilade) is serve as a good source of antioxidants and very useful in medicine field especially in area where it is native such as West Sumatera. This study of antimicrobial and antioxidant activity aims to determine the antimicrobial activity of Halia Merah ethanolic extract using disc diffusion assay and to evaluate the antioxidant activity of Halia Merah in scavenging free radicals. The concentration of Halia Merah ethanolic extract ranging from 400 mg/mL, 200 mg/mL, 100 mg/mL and 50 mg/mL. Based on the result obtained, the highest concentration that is 400 mg/mL showed the largest inhibition zones. The result was 10.00±0.00 mm for S. aureus, 22.75±1.75 mm for E. coli, 28.50±1.06 mm for B. subtilis, and 9.50±1.50 mm for P. aeruginosa. The antioxidant activity that was measured by using DPPH Radical Scavenging Assay determined that the Halia Merah ethanolic extract scavenging activity increased when the concentration is increased; (0.0183 at 50 mg/mL, 0.0397 at 100 mg/mL, 0.0483 at 200 mg/mL, and 0.094 at 400 mg/mL). By using ANOVA analysis, it indicated that the significant difference (p<0.05) across concentrations. As conclusion, the different concentrations of Halia Merah ethanolic extracts have the ability inhibiting the growth of bacteria from Gram-positive and Gram-negative bacteria. Halia Merah ethanolic extract also showed a positive activity of antioxidant by using DPPH Radical Scavenging Assay.