Palm Oil γ-tocotrienol and α-tocopherol Act as Potent Inducers in the Immune Response of Mouse splenocytes in vitro
DOI:
https://doi.org/10.24191/srj.v8i1.5043Keywords:
Gamma-tocotrienol, α-tocopherol, splenocytes, immune responseAbstract
Vitamin E may have anti carcinogenesis effect in human and animal models via the mechanism of cell cycle arrest and enhancement of immune system. The cell-mediated immune inducing properties of palm oil vitamin E, for example, γ-tocotrienol and α-tocopherol, were investigated by measuring the mitogenesis response of splenocytes, extracted from normal male Mus musculus to splenic T-lymphocytes mitogens, phytohemagglutinin (PHA; 0.25 µg/mL) and concanavalin A (Con A; 1.0 µg/mL); and B-lymphocytes mitogen i.e., lipopolysaccharide (LPS; 1.0 µg/mL). Both γ-tocotrienol and α-tocopherol enhanced the cell proliferation of mitogen untreated splenocytes as determined by 5-Bromo-2'-deoxy-uridine (BrdU) detection method. Both compounds also enhanced the T-lymphocytes response to PHA and Con A, as well as B-lymphocytes responses to LPS at all concentration used (0-300 µM). γ-Tocotrienol was observed to affect cell proliferation more than α-tocopherol. The uptake of γ-tocotrienol and α-tocopherol into the splenocytes was determined by high performance liquid chromatography (HPLC). γ-Tocotrienol was absorbed into the cells at markedly higher levels than α-tocopherol with the ratio of 4.8 : 1 (p<0.01, n=4) at 300 µM of treatment. This may be the reason of the higher proliferation affect of γ-tocotrienol as compared to α-tocopherol. In conclusion, we are of the opinion that palm oil γ-tocotrienol and α-tocopherol are able to synergistically influence splenocytes proliferation thus enhancing the cellmediated immune system.
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Copyright (c) 2011 Khalid Abdul Kadir, Narimah Abdul Hamid Hasan, Wan Zurinah Wan Ngah
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